93 research outputs found
Unit Interval Editing is Fixed-Parameter Tractable
Given a graph~ and integers , , and~, the unit interval
editing problem asks whether can be transformed into a unit interval graph
by at most vertex deletions, edge deletions, and edge
additions. We give an algorithm solving this problem in time , where , and denote respectively
the numbers of vertices and edges of . Therefore, it is fixed-parameter
tractable parameterized by the total number of allowed operations.
Our algorithm implies the fixed-parameter tractability of the unit interval
edge deletion problem, for which we also present a more efficient algorithm
running in time . Another result is an -time algorithm for the unit interval vertex deletion problem,
significantly improving the algorithm of van 't Hof and Villanger, which runs
in time .Comment: An extended abstract of this paper has appeared in the proceedings of
ICALP 2015. Update: The proof of Lemma 4.2 has been completely rewritten; an
appendix is provided for a brief overview of related graph classe
Methods of Isolation and Analysis of TREG Immune Infiltrates from Injured and Dystrophic Skeletal Muscle
The immune infiltrate present in acutely injured or dystrophic skeletal muscle has been shown to play an important role in the process of muscle regeneration. Our work has described, for the first time, muscle regulatory T cells (Tregs), a unique population in phenotype and function capable of promoting skeletal muscle repair. Herein, we describe the methods we have optimized to study muscle Tregs, including their isolation from injured muscle, immuno-labeling for analysis/separation by flow cytometry, and measurement of their proliferation status
On the Threshold of Intractability
We study the computational complexity of the graph modification problems
Threshold Editing and Chain Editing, adding and deleting as few edges as
possible to transform the input into a threshold (or chain) graph. In this
article, we show that both problems are NP-complete, resolving a conjecture by
Natanzon, Shamir, and Sharan (Discrete Applied Mathematics, 113(1):109--128,
2001). On the positive side, we show the problem admits a quadratic vertex
kernel. Furthermore, we give a subexponential time parameterized algorithm
solving Threshold Editing in time,
making it one of relatively few natural problems in this complexity class on
general graphs. These results are of broader interest to the field of social
network analysis, where recent work of Brandes (ISAAC, 2014) posits that the
minimum edit distance to a threshold graph gives a good measure of consistency
for node centralities. Finally, we show that all our positive results extend to
the related problem of Chain Editing, as well as the completion and deletion
variants of both problems
Plasticity of the Muscle Stem Cell Microenvironment
Satellite cells (SCs) are adult muscle stem cells capable of repairing damaged and creating new muscle tissue throughout life. Their functionality is tightly controlled by a microenvironment composed of a wide variety of factors, such as numerous secreted molecules and different cell types, including blood vessels, oxygen, hormones, motor neurons, immune cells, cytokines, fibroblasts, growth factors, myofibers, myofiber metabolism, the extracellular matrix and tissue stiffness. This complex niche controls SC biology-quiescence, activation, proliferation, differentiation or renewal and return to quiescence. In this review, we attempt to give a brief overview of the most important players in the niche and their mutual interaction with SCs. We address the importance of the niche to SC behavior under physiological and pathological conditions, and finally survey the significance of an artificial niche both for basic and translational research purposes
Mechanisms of T cell organotropism
F.M.M.-B. is supported by the British Heart Foundation, the Medical Research Council of the UK and the Gates Foundation
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